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. 2019 Sep 4;13:867. doi: 10.3389/fnins.2019.00867

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Copyright © 2019 Duan, Li, Wen, Sunnassee, Chen, Tan and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SMSINS expressed NSC markers similar to NSCs. (A) Neuroepithelial-like colonies of SMSINS at passage 6 (P6) stained with a specific antibody against the NSC marker Nestin (Green). (B) Immunostaining of Nestin (Green) in SMSINS at passage 9 (P9). Nuclei were counterstained with DAPI (blue). (C) Quantitative analyses of the protein expression level of Nestin in SMSINS at passage 0, 6, and 9. Data represent the mean ± SEM. F = 12.39 ^∗p < 0.05; ^∗∗∗p < 0.001 compared with P0 SMSINS; One-way ANOVA followed with Dunnett’s T3 test. (D) SMSINS and native NSCs formed neural spheres when cultured in suspension. (E) Neural spheres of SMSINS stained with specific antibodies against Nestin (Green). Nuclei were counterstained with DAPI (blue). Scale bars: 100 μm.