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. 2019 Sep 4;13:867. doi: 10.3389/fnins.2019.00867

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Copyright © 2019 Duan, Li, Wen, Sunnassee, Chen, Tan and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Three neural lineages differentiation potential of SMSINS in vitro. (A) Differentiation potential of SMSINS without growth factors, identified by immunostaining with antibodies against Tuj1 (Green), NeuN (Red), GFAP (Red), and Olig2 (Red). Nuclei were counterstained with DAPI (blue). (B) Specific differentiation of neurons, astrocytes, and oligodendrocytes from SMSINS, as determined by immunostaining with antibodies against Tuj1 (Green), NeuN (Red), GFAP (Red), and Olig2 (Red). Nuclei were counterstained with DAPI (blue). (C,D) Quantification of neurons, astrocytes, and oligodendrocytes differentiated from SMSINS. All data are represented as the mean ± SEM. Scale bars: 100 μm (A,B).