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. 2019 Sep 10;9:12979. doi: 10.1038/s41598-019-49528-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Exemplary profiles of D₂O concentration (fraction of D₂O to total water) as a function of distance from the root center at selected times after immersion in D₂O during nighttime (a) and daytime (b). D₂O concentration (fraction of D₂O to total water) in the stele and root cortex as a function of time after immersion in D₂O during nighttime (c) and daytime (d). Root stele and the root cortex represent the average concentration in the third innermost and third outermost voxels of the roots. In all figures, the circles show concentrations obtained from image processing of CTs and the lines show the best-simulated profiles of concentration. In (a,c) the region shown with a grey color indicates the position of endodermis.