Figure 1.

Roscovitine- and IPC-mediated neuroprotection against TCI. (A) CV staining in the hippocampus of the TCI (1^st and 2^nd columns), roscovitine + TCI (3^rd and 4^th columns) and IPC + TCI (5^th and 6^th columns) groups. CV⁺ CA1 pyramidal neurons (arrows) are damaged 5 days after TCI; however, CV⁺ CA1 pyramidal neurons (asterisks) in the roscovitine + TCI and IPC + TCI groups are similar to those in the sham group. Scale bar = 800 µm (1^st, 3^rd and 5^th columns), 50 µm (2^nd, 4^th and 6^th columns). (B) NeuN immunohistochemistry (1^st, 3^rd and 5^th columns) and F-J B histofluorescence staining (2^nd, 4^th and 6^th columns) in the CA1 area of the TCI (1^st and 2^nd columns), roscovitine + TCI (3^rd and 4^th columns) and IPC + TCI (5^th and 6^th columns) groups. In the TCI group, a few NeuN⁺ (arrows) and many F-J B⁺ (asterisk) CA1 pyramidal neurons are detected 5 days after TCI. In the roscovitine + TCI and IPC + TCI groups, many NeuN⁺ pyramidal neurons are observed in the CA1 area; F-J B-positive cells are lower than those in the TCI group. SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum. Scale bar = 50 µm. Quantitative graphs for numbers of NeuN⁺ (left) and F-J B⁺ (right) CA1 pyramidal neurons. The bars are reported as means ± SEM from three independent experiments (n = 7, ^*P < 0.05 vs. sham group; ^#P < 0.05 vs TCI group; ^†P < 0.05 vs roscovitine + TCI group).