Figure 13.

suLe^A is present in the luminal epithelium of isthmus and ampulla. Isthmus sections collected from the lower (near the utero-tubal junction, A–C), upper (near the ampullar-isthmic junction, G–I), and medial regions (between the upper and lower junctions, D–F) were labeled with a Le^A/suLe^A antibody (MA17). Ampulla sections were collected from three different regions, the lower (near the ampullary-isthmus junction, J–L), medial (M–O), and upper ampulla (near the infundibulum, P–R). These sections were similarly stained with MA17 antibody. Sections on the left were labeled with MA17 (A, D, G, J, M, and P), those in the middle (competition) were labeled with MA17 preincubated with 25 μg/ml suLe^A (B, E, H, K, N, and Q), and those on the right (control) were prepared without primary antibody (C, F, I, L, O, and R). Scale bar, 20 μm. All of the sections are from a single proestrus/estrus animal and are representative of three animals.