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. 2019 Jul 23;294(36):13445–13463. doi: 10.1074/jbc.RA119.007695

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© 2019 Dutta et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 6. — Neutral loss scan by TIM for detection of sulfated glycans. N-Glycans, GSLs, and O-glycans were analyzed by TIM, and the resulting TIM data were filtered for neutral loss of a sulfate group to identify sulfated glycans. Significant MS signals consistent with sulfation were only detected on glycans released by reductive β-elimination, demonstrating that glycan sulfation is most abundantly presented on O-glycans. The TIM profile for sulfated O-glycans harvested from a representative analysis of bovine oviduct epithelial cells is shown.