Table 1.
Glycan no. | Theoretical mass [M − H]− | Observed mass [M − H]− | Δ mass | Composition | Relative percentage |
---|---|---|---|---|---|
ppm | % | ||||
1 | 576.2325 | 576.2176 | 2.58 | S1Hex1HexNAc1 | 0.75 |
2 | 821.3588 | 821.3366 | 2.70 | S1Hex1HexNAc2 | 22.21 |
3 | 937.4062 | 937.3810 | 2.68 | S1NeuAc1Hex1HexNAc1 | 4.08 |
4 | 954.4215 | 954.3956 | 2.71 | S1deoxyHex1Hex2HexNAc1 | 3.72 |
5 | 995.4480 | 995.4215 | 2.66 | S1deoxyHex1Hex1HexNAc2 | 6.39 |
6 | 1025.4586 | 1025.4305 | 2.74 | S1Hex2HexNAc2 | 15.07 |
7 | 1199.5478 | 1199.5155 | 2.69 | S1deoxyHex1Hex2HexNAc2 | 11.54 |
8 | 1229.5584 | 1229.5261 | 2.62 | S1Hex3HexNAc2 | 4.25 |
9 | 1298.5798 | 1298.5452 | 2.67 | S1NeuAc2Hex1HexNAc1 | 0.51 |
10 | 1328.5904 | 1328.5546 | 2.69 | S1NeuAc1NeuGc1Hex1HexNAc1 | 2.49 |
11 | 1358.6009 | 1358.5522 | 3.59 | S1NeuGc2Hex1HexNAc1 | 1.53 |
12 | 1373.6370 | 1373.6003 | 2.67 | S1deoxyHex2Hex2HexNAc2 | 1.32 |
13 | 1386.6322 | 1386.5951 | 2.68 | S1NeuAc1Hex2HexNAc2 | 2.02 |
14 | 1403.6476 | 1403.6098 | 2.69 | S1deoxyHex1Hex3HexNAc2 | 2.84 |
15 | 1416.6428 | 1416.6041 | 2.73 | S1NeuGc1Hex2HexNAc2 | 7.91 |
16 | 1444.6741 | 1444.6349 | 2.71 | S1deoxyHex1Hex2HexNAc3 | 1.14 |
17 | 1457.6694 | 1457.6278 | 2.85 | S1NeuGc1Hex2HexNAc3 | 1.12 |
18 | 1474.6847 | 1474.6459 | 2.63 | S1Hex3HexNAc3 | 1.03 |
19 | 1560.7215 | 1560.6787 | 2.74 | S1NeuAc1deoxyHex1Hex2HexNAc2 | 1.05 |
20 | 1590.7320 | 1590.6893 | 2.69 | S1NeuGc1deoxyHex1Hex2HexNAc2 | 3.85 |
21 | 1620.7426 | 1620.7000 | 2.63 | S1NeuGc1Hex3HexNAc2 | 1.14 |
22 | 1648.7739 | 1648.7312 | 2.59 | S1deoxyHex1Hex3HexNAc3 | 0.69 |
23 | 1661.7691 | 1661.7257 | 2.61 | S1Neu5Gc1Hex2HexNAc3 | 0.79 |
24 | 1678.7845 | 1678.7398 | 2.66 | S1Hex4HexNAc3 | 0.68 |
25 | 1807.8270 | 1807.7799 | 2.61 | S1Neu5Gc2Hex2HexNAc2 | 0.71 |
26 | 1822.8631 | 1822.8119 | 2.81 | S1deoxyHex2Hex3HexNAc3 | 0.67 |
27 | 1835.8583 | 1835.8109 | 2.58 | S1NeuGcdeoxyHex1Hex2HexNAc3 | 0.34 |
28 | 1865.8689 | 1865.8270 | 2.25 | S1NeuGc12Hex3HexNAc3 | 0.16 |