Figure 5.
Adropin34–76 treatment reduced the expressions of lipogenic genes in the liver. A, triacylglycerol contents were measured and were normalized to tissue masses (n = 8). Real-time RT-PCR was performed to determine the message levels of genes in de novo fatty acid synthesis, including acetyl-CoA carboxylase-α (Acaca) (n = 6), fatty acid synthase (Fasn) (n = 5–6), stearoyl-CoA desaturase (Scd1) (n = 6), and Elovl6 (elongase) (n = 6) (B); de novo TAG synthesis, including mitochondrial glycerol-3-phosphate acyltransferase (Gpam) (n = 6) and diacylglycerol acyltransferase-2 (Dgat2) (n = 6) (C); and acetyl-CoA carboxylase-β (Acacb) (n = 5) (D). *, p ≤ 0.05, adropin versus vehicle Error bars, S.E.