Figure 6.

Adropin^34–76 treatment reduced the nuclear level of SREBP1c in the liver. A, the nuclear levels of SREBP1c (n = 4–5) and the levels of precursor SREBP1c in whole-tissue lysates (n = 4–5) were measured by Western blotting. GAPDH and histone H3 were used as the loading control in the blot of whole-tissue lysates and nuclear lysates, respectively. The same histone H3 band was used as the loading control for the blots of (n)FoxO1 (Fig. 2D), (n)CRTC2 (Fig. 8B), and (n)NF-κB p65 (Fig. S6). B, BiP protein levels in the immunoprecipitates (IP) of precursor SREBP1c from microsomal fractions were determined by Western blotting (IB) (n = 4–5). The blotting was repeated twice, and the blot with 3 samples/treatment was presented. *, p ≤ 0.05; **, p < 0.01, adropin versus vehicle. Error bars, S.E.