Figure 8.

Adropin^34–76 treatment decreased cAMP level and the phosphorylation level of CREB in the liver. A, cAMP contents were measured and were normalized to tissue masses (n = 8). B, the phosphorylation levels of Ser¹³³ in CREB and total CREB levels in whole-tissue lysates (n = 4–5) as well as the nuclear levels of CRTC2 (n = 4–5) were measured by Western blotting. GAPDH and histone H3 were used as the loading control in whole-tissue lysates and nuclear lysates, respectively. The same GAPDH band was used as the loading control for the blot of total IRS2 (Fig. 1B) and the blots of p-c-Jun (Ser⁶³) and total c-Jun (Fig. 4E). The same histone H3 band was used as the loading control for the blots of (n)FoxO1 (Fig. 2D), (n)SREBP1c (Fig. 6A), and (n)NF-κB p65 (Fig. S6). *, p ≤ 0.05, adropin versus vehicle. Error bars, S.E.