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Comparison of effects of single ogc mutants on LPS synthesis and protein glycosylation. Similar results were obtained in three biological repeats. A, proteinase K–treated whole-cell lysates were resolved on 14% Tricine gel and silver staining. Arrowheads indicate a lipid A-core band + truncated O-antigen (see “Results”). B, Western blotting of His-tagged DsbA1 acceptor protein expressed in K56-2 WT, ΔpglL, and Δogc + (OgcE).
