Figure 3.

DEER data of spin-labeled Bax_C87 variants in solution and in membranes. (a) Schemes of possible interspin distances. (b) Primary data with background functions (left) and distance distributions (right) in the monomeric (solution) and oligomeric (membrane-embedded) conformations obtained by one- or two- Gaussian analysis with DeerAnalysis2018⁵⁴. Shaded areas present simulations of intra-dimer distances based on the structures in^18,20. The DEER analysis of the monomeric variants is provided in Supp. Fig. 7. Data evaluation and validation is given in Supp. Fig. 8.