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. 2019 Sep 10;9:13013. doi: 10.1038/s41598-019-49370-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DEER on oligomeric Bax with orthogonal spin labels. (a) Scheme of the expected distances. (b,c) Primary data with background function (left) and distance distributions (right) obtained by Tikhonov regularization with DeerAnalysis2018⁵⁴. The shaded areas present the corresponding distance simulations based on the structures in^18,20. (b) Active Bax_WTR1 mixed with 3-fold excess unlabeled Bax_WT (black) compared to the undiluted Bax_WTR1 (grey). (c) Upper panel, NO-NO DEER on active Bax_WTR1 with 3-fold excess of Bax_C87Gd compared to the Bax_WTR1 alone (grey). Central panel, NO-Gd DEER on the same sample. The asterisk highlights a possible channel cross-talk signal. Bottom panel, Gd-Gd DEER on the same sample (green) compared to Bax_C87Gd alone (grey). Data evaluation and validation is given in Supp. Fig. 9. The same experiments performed with Bax_WTIAG are given in Supp. Figs 10, 11.