Figure 1.

Generation of the Ryr1^TM/Indel mouse line. (A) Using CRISPR/Cas9, a threonine-to-methionine missense mutation (T4709M) and a 16 bp deletion (Indel) were generated in exon 96 of the mouse Ryr1 locus. ‘y’ indicates a heterozygous base change from C to T. (B) Representative Sanger sequencing results showing the T4709M substitution and 16 bp deletion mutations. (C) Breeding strategy of crossing heterozygous Ryr1^WT/TM mice with Ryr1^WT/Indel mice to obtain compound Ryr1^TM/Indel mice.