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. 2019 Sep 4;13:920. doi: 10.3389/fnins.2019.00920

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Copyright © 2019 Chen, Zhao, Sun, Wang and Ni.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mossy fiber sprouting in CA3 and dentate gyrus subfields by Timm staining. Developmental seizures significantly increased the Timm particle scores in the hippocampal CA3 and DG subfields of rats in SE, SE + ZD, and SE + ZS groups compared to corresponding Control, ZD and ZS groups, respectively. Moreover, the Timm particle scores were significantly higher in SE + ZD group compared to the SE group. Further, Timm particle scores were significantly lower in the SE + ZS group than in the SE group (one-way ANOVA with post hoc tests, ^∗P < 0.05, compared to Control group, #P < 0.05 compared to ZD group, ΔP < 0.05 compared to ZS group, a P < 0.05 compared to SE group, n = 4/group).