TABLE 1.
Variation partitioning of the long-term effect of tillage and nitrogen fertilization on soil prokaryotic and fungal diversity at different phylogenetic resolution and two soil depths, in a wheat-soybean rotation in the Mediterranean area.
|
0–15 cm |
15–30 cm |
|
| Variance explained | Variance explained | |
| Prokaryotes at class level | ||
| TILa | 1.96b | – |
| N fert | 22.08 | – |
| TIL × N fert | 50.15 | – |
| Prokaryotes at phylum level | ||
| TIL | 8.38 | – |
| N fert | 24.19 | – |
| TIL × N fert | 29.72 | – |
| Fungi at family level | ||
| TIL | 25.26 | 24.20 |
| N fert | 18.36 | 14.81 |
| TIL × N fert | 22.89 | 27.14 |
| Fungi at phylum level | ||
| TIL | 46.76 | 32.67 |
| N fert | 6.94 | −7.69 |
| TIL × N fert | 33.88 | −8.02 |
aPermutational analyses of variance were performed following a split-plot design with tillage (TIL) as main-plot factor and nitrogen fertilization (N fert) as subplot factor and with three replicate plots per treatment: TIL (minimum tillage and conventional tillage) and N fert (0 kg N ha–1 and 200 kg N ha–1). bIn bold statistically significant values (P ≤ 0.05).