Table 3.
Validated miRNA target genes and primer sequence.
| miRNA | Target gene ID | Primer sequence (5’–3’) | Target annotation |
|---|---|---|---|
| novel_miR692-5p | 3B_220641_AA0713280.1 (Traes_3B_E1131EA03.1) |
Rev1: AACACCAATCTCCGTCCGTC | Integrator complex subunit 9 homolog |
| Rev2: AGTAGAATCGATCGCTACAAC | |||
| novel_miR84 | 2AS_113055_AA0350560.2 (Traes_2AS_6BF19AB61.1) |
Rev1: ACAGACCGTATGCTAGCAATG | Beta-galactoside alpha-2,3-sialyltransferase |
| Rev2: ACACTCCTGTGTTCAGCCTC | |||
| tae-miR1127a | 5BS_423630_AA1380910.2 | Rev1: ACTGCACGATATTTGGATGCTTAAC | Putative disease resistance protein RGA1 |
| Rev2: TATTCTTGAGTCCACTGAGAATCC | |||
| tae-miR171a-3p | 6AL_472660_AA1524550.1 (Traes_6DL_26DDCA106.1) |
Rev1: AGTACCACGGCGATGAGCAAG | Scarecrow-like protein 6 |
| Rev2: AGGCGATTTCGTCGAGAAGC | |||
| tae-miR172e-3p | 2AL_097448_AA0324210.1 (Traes_2AL_5BA7E2623.1) |
Rev1: ACCACATCCATCCGTCCTCTC | AP2-like ethylene-responsive TF |
| Rev2: AGTTCCAGTTGAGTTGTGGTG | |||
| tae-miR5181-3p | 4DL_343545_AA1136340.2 (Traes_4DL_9294C72F2.1) |
Rev1: AGCGAGTACACATATACAGGACAG | vulgare mRNA for predicted protein |
| Rev2: TAACAGGAGGCAAAATGCCG | |||
| tae-miR9662a-3p | 6DS_542718_AA1728780.1 (Traes_6DS_F6C7E588F.1) |
Rev1: AGCTCAAGTCACGATCTAGCAG | Transcription termination factor MTERF15 |
| Rev2: TGATCGATGAGCAATGTACACG | |||
| tae-miR9674b-5p | 6BS_513439_AA1641660.1 | Rev1: TTACCTTCTGCAGCCTATGAC | Protein Rf1, mitochondrial-like |
| Rev2: AGCTCTGTCATGAGGTACCCTG |
3B_220641_AA0713280.1 is a short name of TRIAE_CS42_3B_TGACv1_220641_AA0713280.1 in ftp://ftp.ensemblgenomes.org/pub/plants/release-37/fasta/triticum_aestivum/; the same abbreviation applies to other target gene IDs. Gene ID in parenthood is the gene ID in ftp://ftp.ensemblgenomes.org/pub/plants/release-31/fasta/triticum_aestivum/.