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. 2019 Sep 3;28(10):2673–2688.e8. doi: 10.1016/j.celrep.2019.07.104

Figure 3.

Figure 3

Distinct DNA Binding States around the MCM Ring in the Translocating CMG

(A) Four distinct DNA binding states for the translocating CMG. In states 2B, 2A, and 1A, four ATPase protomers contain DNA-interacting pore loops arranged as a staircase. In state 1B only three protomers contact DNA forming a pore-loop spiral. Both helix 2 insertion (h2i) as well as Pre-sensor 1 beta hairpin (PS1h) pore loops contact DNA, resulting in two nucleotide contacts per protomer.

(B) Detail of the cryo-EM density assigned to DNA shows that individual phosphate groups can be resolved in the single-stranded DNA region of the DNA fork, allowing us to count the number of nucleotides contacted by each ATPase protomer.