Table 2.

Enzymatic activity of the salt correctible lacZ and xylE missense mutants in the presence of 0.2 M or 0.3 M NaCl

Mutant	0.0 M NaCl	0.2 M NaCl	0.2 M NaCl Fold Increase	0.3 M NaCl	0.3 M NaCl Fold Increase
β-galactosidase
lacZ39	1.29	25.39	19.68	34.24	26.54
lacZ337	0.65	1.03	1.58	0.90	1.38
lacZ364	0.48	1.04	2.17	0.86	1.79
lacZ2234	0.70	0.89	1.27	0.91	1.30
lacZ2343	14.28	77.96	5.46	87.54	6.13
lacZ2381	0.78	2.37	3.04	2.67	3.42
lacZ2396	0.24	0.43	1.79	0.31	1.29
lacZ2449	1.26	14.27	11.33	12.41	9.85
lacZ2456	1.20	10.07	8.39	10.18	8.48
lacZ2530	0.79	2.42	3.06	2.61	3.30
lacZ2540	0.75	2.26	3.01	2.24	2.99
Catechol 2,3-dioxygenase
xylE1568	7.46	10.40	1.39	14.17	1.90
xylE1569	20.59	25.69	1.25	30.58	1.49
xylE1583	8.34	11.24	1.35	11.82	1.42
xylE1585	8.17	17.67	2.16	15.00	1.84
xylE1591	14.16	20.34	1.44	26.75	1.89
xylE1683	6.76	16.82	2.49	11.77	1.74
xylE1698	7.42	10.41	1.40	15.56	2.10
xylE1702	9.60	14.14	1.47	21.09	2.20
xylE1710	6.18	10.92	1.77	11.61	1.88

β-galactosidase activities are reported in Miller Units and catechol 2,3-dioxygenase activities are reported in XylE Units. All assays were repeated in triplicate and the standard deviation was less than 10%.