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Immunohistochemical staining of CSC markers by MCF-7 cells in spheroid culture. MCTS: (A) Bmi-1, (B) CD44, (C) CD133, (D) CD24; eMCTS: (E) Bmi-1, (F) CD44, (G) CD133, (H) CD24; magnification ×100, hematoxylin, immunopositive cells [black arrows (PolyVueHRP/DAB Diagnostic BioSystems, USA)]. CSC, cancer stem cell; MCTS, multicellular tumor spheroids.
