Figure 2.

Dual hSEAP and hFIX transgene protein expression only in NHPs following IA. NHPs received a primary administration of rAAV5-hSEAP (day 0) and a secondary readministration of rAAV5-hFIX (day 49) with or without prior IA treatment (IA). (A) hSEAP protein expression was stable throughout the experiment and not impacted by the IA procedure. (B) hFIX protein was expressed successfully after IA. As a reference for hFIX levels, 2 NHPs that were administered with 3 × 10¹³ gc/kg rAAV5-hFIX were included. (C) The averages of the areas under the curves (AUC) ± standard deviation calculated for the hFIX expression over time showed an hFIX increase for the group submitted to IA (NHPs 4-6) when compared with the group without IA (NHPs 1-3). Furthermore, a decrease of 2.9 times was observed for the NHPs submitted to IA (NHPs 4-6) compared with the control animals (NHPs controls 1 and 2). RLU, relative light unit.