Table 1.
Integrative list of deformation-induced phosphorylations in the reticulocyte
| Accession no. | Gene symbol | Protein name | Sequence | Count | Site |
|---|---|---|---|---|---|
| E9PIJ1 | AMPD2 | AMP deaminase 2 | TDSDSDLQLYK | 7* | S190/S197 |
| A0A0U1RQT1 | ACAP2 | Arf-GAP with coiled-coil, ANK repeat, and PH domain-containing protein 2 | SSPSTGSLDSGNESK | 7* | S384 |
| B7Z3I9 | ALAD | Δ-aminolevulinic acid dehydratase | SSPAFGDR | 7 | S215 |
| P49840 | GSK3A | Glycogen synthase kinase-3 α | GEPNVSYICSR | 7* | Y279 |
| P49840 | GSK3A | Glycogen synthase kinase-3 α | TSSFAEPGGGGGGGGGGPGGSASGPGGTGGGK | 7* | S21/S39 |
| E9PK89 | RAB3IL1 | Guanine nucleotide exchange factor for Rab-3A | TLVITSTPASPNR | 7 | S168 |
| B4DMA2 | HSP90AB1 | Heat shock protein HSP 90-β | IEDVGSDEEDDSGKDK | 7* | S255 |
| Q9H1E3 | NUCKS1 | Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1 | VVDYSQFQESDDADEDYGR | 7* | S19 |
| S4R2Y4 | CBX3 | Chromobox protein homolog 3 | SLSDSESDDSK | 6 | S93 |
| Q08495 | DMTN | Dematin | DSSVPGSPSSIVAK | 6 | S26 |
| Q9UG54 | MAP3K7 | Mitogen-activated protein kinase kinase kinase 7 | SIQDLTVTGTEPGQVSSR | 6 | S439 |
| P61006 | RAB8A | Ras-related protein Rab-8A | KLEGNSPQGSNQGVK | 6 | S181/S185 |
| B3KVN0 | SLC2A1 | Solute carrier family 2, facilitated glucose transporter member 1 | TPEELFHPLGADSQV | 6 | S490 |
| A0A087WYB4 | STOML2 | Stomatin-like protein 2, mitochondrial | APVPGTPDSLSSGSSR | 6 | T327 |
| Q8IV54 | TSC22D4 | TSC22D4 protein | NGSPPPGAPSSR | 6 | S62 |
| P16157 | ANK1 | Ankyrin-1 | NGASPNEVSSDGTTPLAIAK | 5 | S759 |
| A0A0U1RQT1 | ACAP2 | Arf-GAP with coiled-coil, ANK repeat, and PH domain-containing protein 2 | YSISLSPPEQQK | 5 | S521 |
| P27824 | CANX | Calnexin | QKSDAEEDGGTVSQEEEDR | 5 | S564 |
| G3V238 | EEF1AKMT2 | EEF1A lysine methyltransferase 2 | SDKGSPGEDGFVPSALGTR | 5 | S21 |
| P05198 | EIF2S1 | Eukaryotic translation initiation factor 2 subunit 1 | VVTDTDETELAR | 5 | T279 |
| F8WAE5 | EIF2A | Eukaryotic translation initiation factor 2A | SDKSPDLAPTPAPQSTPR | 5 | S506 |
| C9JII4 | GCFC2 | GC-rich sequence DNA-binding factor 2 | ELPVPGSAEEEPPSGGGR | 5 | S40 |
| Q2VPJ6 | HSP90AA1 | HSP90AA1 protein | DKEVSDDEAEEK | 5 | S231 |
| D3DWY2 | IKBKG | Inhibitor of κ light polypeptide gene enhancer in B-cells, kinase γ | SPPEEPPDFCCPK | 5 | S387 |
| O60307 | MAST3 | Microtubule-associated serine/threonine-protein kinase 3 | SSENVLDEEGGR | 5 | S134 |
| F2Z2K0 | NSFL1C | NSFL1 cofactor p47 | SPNELVDDLFK | 5 | S114 |
| B7Z8Y1 | NFATC2 | Nuclear factor of activated T-cells, cytoplasmic 2 | TSPDPSPVSAAPSK | 5 | S330 |
| H0Y5Q9 | INPP5D | Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 | GESPPTPPGQPPISPK | 5 | T963 |
| D6RC77 | PGM3 | Phosphoacetylglucosamine mutase | STIGVMVTASHNPEEDNGVK | 5 | S64 |
| M4QHP2 | PIEZO1 | Piezo-type mechanosensitive ion channel component | SGSEEAVTDPGER | 5 | S1621 |
| Q9BVG4 | PBDC1 | Protein PBDC1 | GADSGEEKEEGINR | 5 | S197 |
| B4E3V2 | SQSTM1 | Sequestosome-1 | LTPVSPESSSTEEK | 5 | T269/S272 |
| Q6PJD5 | SHARPIN | SHARPIN protein | SPGNLTER | 5 | S165 |
| B2RMN7 | SPTB | Spectrin β chain | TSPVSLWSR | 5 | S2114/S2117 |
| C9JWF0 | SMC4 | Structural maintenance of chromosomes protein 4 | REEGPPPPSPDGASSDAEPEPPSGR | 5 | S22 |
| H0YN01 | TLN2 | Talin-2 | LDEGTPPEPK | 5 | T1843 |
| C9J3F6 | TBC1D5 | TBC1 domain family member 5 | NISSSPSVESLPGGR | 5 | S541/S544 |
| E9PJJ0 | TBCEL | Tubulin-specific chaperone cofactor E-like protein | YSPENFPYR | 5 | S18 |
| Q8IXQ3 | C9orf40 | Uncharacterized protein C9orf40 | RDSGDNSAPSGQER | 5 | S76 |
| Q5JSH3 | WDR44 | WD repeat-containing protein 44 | VGNESPVQELK | 5 | S50 |
| F8WBS8 | PSMD2 | 26S proteasome non-ATPase regulatory subunit 2 | DKAPVQPQQSPAAAPGGTDEKPSGK | 4 | S16 |
| Q92625 | ANKS1A | Ankyrin repeat and SAM domain-containing protein 1A | SESLSNCSIGK | 4 | S647 |
| E9PJP4 | EPB41L2 | Band 4.1-like protein 2 | EVAENQQNQSSDPEEEKGSQPPPAAESQSSLR | 4 | S39/S58 |
| A8MZ77 | BSG | Basigin | RKPEDVLDDDDAGSAPLK | 4 | S362 |
| Q9NSY1 | BMP2K | BMP-2-inducible protein kinase | DSQSSNEFLTISDSK | 4 | S1029 |
| A0A0C4DGD1 | CAST | Calpastatin | DTSQSDKDLDDALDK | 4 | S563 |
| Q9Y5K6 | CD2AP | CD2-associated protein | KNSLDELR | 4 | S582 |
| Q8TEH3 | DENND1A | DENN domain-containing protein 1A | TGGTLSDPEVQR | 4 | T24 |
| B4DU91 | EPN1 | Epsin-1 | SPGAFDMSGVR | 4 | S435 |
| M0QXW7 | KHSRP | Far upstream element-binding protein 2 | VQISPDSGGLPER | 4 | S181 |
| F5H1C6 | FERMT3 | Fermitin family homolog 3 | TASGDYIDSSWELR | 4 | Y11 |
| P04075 | ALDOA | Fructose-bisphosphate aldolase A | GILAADESTGSIAKR | 4 | S36 |
| H0YJ30 | GPHN | Gephyrin | ASHSAVDITK | 4 | S303/S305 |
| Q2Q9B7 | G6PD | Glucose-6-phosphate 1-dehydrogenase | VQPNEAVYTK | 4 | Y401 |
| E9PK89 | RAB3IL1 | Guanine nucleotide exchange factor for Rab-3A | ELHPQLLSPTK | 4 | S179 |
| O75633 | GNAS | Guanine nucleotide-binding protein Gs α subunit isoform L3 | ISTASGDGR | 4 | S352 |
| Q5H924 | HUWE1 | HECT, UBA, and WWE domain containing 1 | REESPMDVDQPSPSAQDTQSIASDGTPQGEK | 4 | S3816 |
| A0A0F7NGI8 | LRRFIP1 | Leucine rich repeat | IDGATQSSPAEPK | 4 | S714 |
| E9PJF4 | CLNS1A | Methylosome subunit pICln | EPVADEEEEDSDDDVEPITEFR | 4 | S102 |
| S4R3D6 | APIP | Methylthioribulose-1-phosphate dehydratase | DISGPSPSKK | 4 | S87 |
| Q6NT16 | SLC18B1 | MFS-type transporter SLC18B1 | SKSQNILSTEEER | 4 | S438 |
| B4DS42 | OATL1 | Ornithine aminotransferase-like 1 | QASLDGLQQLR | 4 | S506 |
| P30041 | PRDX6 | Peroxiredoxin-6 | DFTPVCTTELGR | 4 | T44 |
| I3L1U0 | RILP | Rab-interacting lysosomal protein | AESSEDETSSPAPSK | 4 | S354 |
| Q9P227 | ARHGAP23 | ρ GTPase-activating protein 23 | SAEALGPGALVSPR | 4 | S361/S372 |
| Q16513 | PKN2 | Serine/threonine-protein kinase N2 | ASSLGEIDESSELR | 4 | S583 |
| C9JIG9 | OXSR1 | Serine/threonine-protein kinase OSR1 | TEDGGWEWSDDEFDEESEEGK | 4 | S339 |
| P02549 | SPTA1 | Spectrin α chain, erythrocytic 1 | QDTLDASLQSFQQER | 4 | S1976 |
| B2RMN7 | SPTB | Spectrin β chain | LSSSWESLQPEPSHPY | 4 | S2123/S2125/S2128 |
| B4DZC9 | SLK | STE20-like serine/threonine-protein kinase | VDEDSAEDTQSNDGK | 4 | S571 |
| P17987 | TCP1 | T-complex protein 1 subunit α | HGSYEDAVHSGALND | 4 | S544/Y545 |
| B4DUI5 | TPI1 | Triosephosphate isomerase | IIYGGSVTGATCK | 4 | Y246 |
| Q8IV54 | TSC22D4 | TSC22D4 protein | VEAEAGGSGARTPPLSR | 4 | T229 |
| B4DQ79 | LYN | Tyrosine-protein kinase | VIEDNEYTAR | 4 | Y397 |
| A0A024R8I2 | UBADC1 | Ubiquitin associated domain containing 1, isoform CRA_c | APSPLPK | 4 | S98 |
| Q5VVQ6 | YOD1 | Ubiquitin thioesterase OTU1 | SSPAFTK | 4 | S130 |
| B3KW31 | VPS13A | Vacuolar protein sorting 13A | QASFTDVRDPSLK | 4 | S1416 |
| A0A0S2Z3K9 | CRK | V-crk sarcoma virus CT10 oncogene-like protein isoform 2 | DSSTSPGDYVLSVSENSR | 4 | S41 |
List of significantly enriched or exclusive phosphopeptides in reticulocytes after deformation, as assayed through microsphiltration and microcapillary traversal. Generally, only phosphopeptides present in at least 4 of the 7 postdeformation samples (4 microsphiltration + 3 microfluidics) and not present in at least 4 of the 7 predeformation samples were included. The exception to this analysis consists of the phosphopeptides counted as 7*, which were present in all postdeformation samples but also in 4 of 7 of the predeformation samples. The prediction of the most probable phosphosites in the phosphopeptide was included according to the count of occurrences in previous low-throughput and high-throughput studies, as assayed through PhosphoSitePlus.50