. 2019 Sep 4;10:2002. doi: 10.3389/fmicb.2019.02002

TABLE 2.

Basic information of the twelve bla_NDM–₅ positive E. coli strains revealed by WGS data.

									Number of
		MLST		Conjugation		MinION	Plasmid names	Size	resistance	Accession
Strains	Species	types	Source	Transferability^a	Resistance genes	sequencing	(or replicons)	(bp)	genes	numbers
L37	Escherichia coli	ST-48	Anal swab	+	fosA, sul2, aac(3)-IId, strA, strB, qnrS1, dfrA14, tet(A), floR, bla_{CTX–M–55,} bla_NDM–5, bla_TEM–1B, aph(6)-Id, aph(3″)-Ib	+	pL37-2 (IncFIB, p0111)	145704	9	CP034590
							pL37-3 (IncX3)^d	45650	1	CP034591
							pL37-4 (IncFII)	76559	3	CP034592
L41-1	Escherichia coli	ST-48	Feed	+	strA, strB, sul2, floR, bla_{CTX–M–64,} bla_NDM–5, bla_TEM–1A, tet(A)	+	pL41-1-2 (IncFIB, IncHIB, p0111)	201021	8	CP034728
							pL41-1-3 (IncFIB)	111458	1	CP034729
							pL41-1-4 (IncX3)^d	46238	1	CP034730
L53	Escherichia coli	ST-48	Water	+	fosA, sul2, strA, strB, aac(3)-IId, qnrS1, dfrA14, tet(A), floR, bla_TEM–1B, bla_NDM–5, bla_CTX–M–55	+	pL53-2 (IncFIB, p0111)	137848	9	CP034745
							pL53-3 (IncFII)	76642	3	CP034756
							pL53-4 (IncX3)^d	46259	1	CP034757
L65	Escherichia coli	ST-3076	Feces	+	tet(A), qnrS1, dfrA14, floR, bla_TEM–1C, bla_NDM–5	+	pL65-2 (IncFIB, IncFII)	145346	6	CP034739
							pL65-9 (IncX3)^d	46161	1	CP034744
L100	Escherichia coli	ST-48	Anal swab	+	strA, strB, sul2, floR, bla_{CTX–M–64,} bla_TEM–1A, bla_NDM–5, tet(A)	+	pL100-2 (IncFIB, IncHIB, p0111)	202387	8	CP034746
							pL100-3 (IncFIB)	111458	1	CP034747
							pL100-4 (IncX3)^d	46261	1	CP034748
L103-2	Escherichia coli	ST8809	Anal swab	+	arr-2, rmtB, aph(3′)-IIa, aadA1, fosA, dfrA14, tet(A), cmlA1, floR, bla_TEM–1B, bla_NDM–5, bla_OXA–10, bla_CTX–M–55	+	pL103-2-2 (IncFIB)	110210	0	CP034844
							pL103-2-3 (IncFIB)	102295	1	CP034845
							pL103-2-4 (IncFII)	100667	6	CP034846
							pL103-2-5 (IncX3)^d	46163	1	CP034847
							pL103-2-6 (IncX1)	13362	1	CP034848
							pL103-2-7 (unknown)	12606	6	CP034849
L33	Escherichia coli	ST-48	Anal swab	+	strB, strA, aac(3)-IId, sul2, fosA, qnrS1, dfrA14, tet(A), floR, bla_NDM–5, bla_{CTX–M–55,} bla_TEM–1B	–	IncX3^d, p0111	46kb^c	12	Online^b
L43-1	Escherichia coli	ST-48	Feces	+	strA, strB, sul2, floR, bla_{CTX–M–64,} bla_TEM–1A, bla_NDM–5, tet(A)	–	IncX3^d, p0111, colRNAI	46kb	8	Online
L56	Escherichia coli	ST-48	Water	+	fosA, sul2, strA, strB, aac(3)-IId, qnrS1, dfrA14, tet(A), floR, bla_TEM–1B, bla_NDM–5, bla_CTX–M–55	–	IncX3^d, p0111	46kb	12	Online
L99	Escherichia coli	ST-48	Anal swab	+	fosA, sul2, strA, strB, aac(3)-IId, qnrS1, dfrA14, tet(A), floR, bla_NDM–5, bla_{CTX–M–55,} bla_TEM–1B	–	IncX3^d, p0111	46kb	12	Online
L102	Escherichia coli	ST-48	Anal swab	+	tet(A), sul2, bla_{CTX–M–64,} bla_NDM–5, strB, strA	–	IncX3^d, p0111	46kb	6	Online
L103-1	Escherichia coli	ST-48	Anal swab	+	strB, strA, aac(3)-IId, sul2, fosA, qnrS1, dfrA14, tet(A), floR, bla_NDM–5, bla_TEM–1B, bla_CTX–M–55	–	IncX3^d, p0111, colRNAI	46kb	12	Online

^aTransference of plasmids carrying bla_NDM–₅. ^bThe assembly results of six strains without complete genome sequences were deposited in figshare database for reference (https://figshare.com/articles/L33-L103-1/8044847). ^cThe length of bla_NDM–₅-bearing IncX3 plasmids in samples without MinION sequencing were infered from S1-PFGE. ^dbla_NDM–₅-bearing plasmid.