Table 2.
Primer sequences of fluorescence quantitative PCR.
| Target | Accession No. | Forward (5′ to 3′) | Reverse (5′ to 3′) |
|---|---|---|---|
| B0AT | XM_419056.4 | GGGTTTTGTGTTGGCTTAGGAA | TCCATGGCTCTGGCAGAGAT |
| ATB0,+ | XM_004940661.1 | TGGCAACATCGTGTGGTACCT | AGGCAGCTCCAACGATCATC |
| b0,+AT | NM_004935075.1 | CAGTAGTGAATTCTCTGAGT GTGAAGCT | GCAATGATTGCCAC AACTACCA |
| SAT1 | NM_001199603.1 | CACAGTGCCAGTGCTGTTTTT C | TGTTTTTCTTGCCAGCTCGAA |
| LAT1 | NM_001030579.1 | GATTGCAACGGGTGATGTGA | CCCCACACCCACTTTTGTTT |
| y+LAT2 | XM_001231336.3 | GCCCTGTCAGTAAATCAGACA AGA | TTCAGTTGCATTGTGTTT TGGTT |
| MCT1 | NM_001006323.1 | AGCAGCATCCTGGTGAACAAG | AGGCACCCACCCACGAT |
| NHE3 | XM_001199133.1 | AGGCTGGACCGGTTTGC | TCCCGAATACTTTTCCTCCTTTG |
| MAT1 | NM_001199519.1 | CCAGGTTAGCAGAGCTGAGG | ATGGACACGCACTGGTATGA |
| MrsA | XM_004935891.2 | CTGGCCACACAGAGGTTGTA | AGTCATTACCTTGCCGCATT |
| Trx | NM_205453.1 | GATTTCTCTGCCACATGGTGT | ATCTTGGGCATCATCCACAT |
| Grx | NM_205160.1 | CCGTCCCTCGTGTGTTTATT | CACCAGAGCACCAATTTGAC |
| GSR | XM_015276627.1 | GTGGTTACGGCAAGTTCACC | CACAGTCAGGAGGGACCACT |
| GSS | XM_425692.5 | TTGCTGGGCTGTACTCACTG | ACAGGTTGTTCCCTCCTCCT |
| GAPDH | K01458 | TGCTGCCCAGAACATCATCC | ACGGCAGGTCAGGTCAACAA |
B0AT = Na+ dependent neutral amino acid transporter; ATB0, + = Na+ and Cl− dependent neutral and cationic amino acid transporter; b0, +AT = Na+-independent cationic and zwitterionic amino acid transporter; SAT1 = diamine acetyltransferase 1; LAT1 = Na+-independent branched-chain and aromatic amino acid transporter; y+LAT2 = Na + -independent cationic & Na+-dependent neutral amino acid; MCT1 = proton dependent monocarboxylate transporter 1; NHE3 = Na+-H+ exchanger; MAT1 = methionine adenosyltransferase 1; MrsA = methionine sulfoxide reductase A; Trx = thioredoxin; Grx = glutaredoxin; GSR = glutathione reductase; GSS = glutathione synthetase; GAPDH = glyceraldehyde-3-phosphate dehydrogenase.