Table 4.
Source | Level, % | Serum |
Liver |
||||
---|---|---|---|---|---|---|---|
GPx, U/mL | GPx, U/mg protein | T-GSH, nmol/g | GSSG, nmol/g | GSH, nmol/g | GSH:GSSG | ||
DLM | 0.05 | 1,665.89 | 30.74 | 1,733.23a | 428.81a | 875.61 | 4.10 |
0.25 | 2,639.79 | 34.96 | 2,132.34b | 635.82b | 860.70 | 3.35 | |
HMTBa | 0.05 | 1,699.77 | 32.47 | 1,512.09a | 423.15a | 665.79 | 3.63 |
0.25 | 2,457.07 | 35.63 | 1,720.89a | 422.82a | 715.79 | 3.32 | |
SEM | 59.22 | 0.80 | 63.37 | 23.94 | 32.80 | 0.06 | |
Main effect | |||||||
Source | DLM | 2,152.84 | 33.35 | 1,932.79b | 532.32b | 868.15b | 3.73 |
HMTBa | 2,078.42 | 33.93 | 1,475.47a | 427.66a | 620.14a | 3.48 | |
Level, % | 0.05 | 1,682.83a | 31.99 | 1,622.66 | 425.98a | 770.70 | 3.86b |
0.25 | 2,548.43b | 35.29 | 1,785.60 | 534.00b | 717.60 | 3.35a | |
P-value | |||||||
Source | 0.540 | 0.724 | <0.001 | 0.002 | <0.001 | 0.079 | |
Level | <0.001 | 0.054 | 0.095 | 0.002 | 0.332 | 0.001 | |
Source × Level | 0.374 | 0.955 | 0.019 | 0.004 | 0.484 | 0.100 |
GPx = glutathione peroxidase; T-GSH = total glutathione; GSSG = glutathione disulfide; GSH = glutathione; DLM = DL-methionine; HMTBa = DL-2-hydroxy-4-methylthio-butanoic acid.
a, b Within a column, means sharing the same superscripts are not significantly different from each other at P < 0.05.