Table 6.

Effects of 2 sources and levels of methionine on the antioxidant-related gene expression in intestine and liver of broilers on d 21.

Source	Level, %	Duodenum						Jejunum						Ileum						Liver
		MAT1	MrsA	Trx	Grx	GSR	GSS	MAT1	MrsA	Trx	Grx	GSR	GSS	MAT1	MrsA	Trx	Grx	GSR	GSS	MAT1	MrsA	Trx	Grx	GSR	GSS
DLM	0.05	1.02	1.03	0.74	0.89	1.21	1.13	1.14	0.96	1.05	1.06	0.94	1.03	1.01	1.02	1.01	1.01a	1.03	1.05	1.03	1.06	1.02b	1.07	1.03	1.06
	0.25	0.98	0.78	0.72	0.74	1.19	0.97	0.96	1.06	0.85	0.96	1.02	1.14	0.91	0.98	0.97	0.87a	1.02	1.14	0.81	1.01	0.73a	0.67	0.90	0.90
HMTBa	0.05	0.71	0.90	0.79	0.81	1.38	1.18	1.24	0.86	0.97	0.78	1.02	1.09	1.05	1.00	1.07	1.06ab	1.18	1.36	1.25	1.31	0.86ab	2.12	0.97	0.84
	0.25	0.95	1.04	0.94	0.86	1.47	1.35	1.16	1.11	1.07	0.99	1.22	1.37	0.74	1.28	1.32	1.37b	1.37	1.56	1.11	1.10	0.95b	0.65	1.23	1.20
SEM								0.09	0.05	0.06	0.07	0.06	0.07	0.05	0.05	0.05	0.06	0.06	0.07	0.06	0.04	0.04	0.17	0.06	0.07
Main effect
Source	DLM	1.07	0.84	0.72a	0.81	1.30	1.12	1.20	0.96	0.88	0.86	0.94	0.97	0.94	0.97	0.94a	0.92a	0.97a	1.04a	0.94a	1.04	0.90	0.83	0.99	1.00
	HMTBa	0.81	0.96	0.86b	0.83	1.49	1.32	1.18	0.93	0.98	0.83	1.06	1.12	0.89	1.16	0.89b	1.26b	1.28b	1.48b	1.16b	1.16	0.90	1.23	1.14	0.97
Level, %	0.05	0.91	0.91	0.76	0.80	1.43	1.29	0.92	1.35	0.95	0.82	0.94	1.01	1.02	0.98	1.01	1.02	1.05	1.15	1.17b	1.14	0.97	1.41b	1.06	1.00
	0.25	0.96	0.89	0.82	0.85	1.36	1.16	0.97	1.04	0.90	0.86	1.05	1.08	0.81	1.15	1.14	1.17	1.20	1.37	0.93a	1.06	0.83	0.64a	1.07	0.97
P-value
Source		0.135	0.256	0.032	0.833	0.340	0.258	0.904	0.719	0.480	0.828	0.325	0.174	0.583	0.058	0.016	0.008	0.018	0.003	0.047	0.078	0.920	0.151	0.185	0.800
Level		0.754	0.818	0.357	0.617	0.719	0.442	0.125	0.652	0.725	0.738	0.349	0.535	0.051	0.089	0.150	0.211	0.247	0.106	0.044	0.201	0.055	0.009	0.939	0.808
Source × Level		0.552	0.079	0.129	0.927	0.078	0.053	0.341	0.481	0.924	0.939	0.624	0.596	0.198	0.137	0.181	0.042	0.732	0.902	0.820	0.568	0.005	0.102	0.106	0.220

MAT1 = methionine adenosyltransferase 1; MrsA = methionine sulfoxide reductase A; Trx = thioredoxin; Grx = glutaredoxin; GSR = glutathione reductase; GSS = glutathione synthetase; DLM = DL-methionine; HMTBa = DL-2-hydroxy-4-methylthio-butanoic acid.

^{a, b} Within a column, means sharing the same superscripts are not significantly different from each other at P < 0.05.