. 2019 Sep 11;12:448. doi: 10.1186/s13071-019-3699-x

Table 1.

Selected primer pairs and annealing temperature for the detection of mitochondrial target regions for the genera Babesia/Theileria, Anaplasma/Ehrlichia, Rickettsia and Borrelia

Genus	Primer	Target gene	Primer sequence (5′-3′)	Annealing T (°C)	Amplicon size (bp)	References
Babesia/Theileria	RLB-F2	18S rDNA	GACACAGGGAGGTAGTGACAAG	57	460–500	[12]
Babesia/Theileria	RLB-R2	18S rDNA	CTAAGAATTTCACCTCTGACAGT	57	460–500	[12]
Anaplasma/Ehrlichia	AnaEhr16S_f	16S rDNA	AGAGTTTGATCMTGGYTCAGAA	55	460–520	This study
Anaplasma/Ehrlichia	Ana-Ehr16S_r	16S rDNA	GAGTTTGCCGGGACTTYTTC	55	460–520	This study
Rickettsia	Rick-F1	16S rDNA	GAACGCTATCGGTATGCTTAACACA	64	350–400	[13]
Rickettsia	Rick-F2	16S rDNA	CATCACTCACTCGGTATTGCTGGA	64	350–400	[13]
Borrelia outer	16S1A	16S rDNA	CTAACGCTGGCAGTGCGTCTTAAG	63	1205	[14]
Borrelia outer	16S1B	16S rDNA	AGCGTCAGTCTTGACCCAGAAGTT	63	1205	[14]
Borrelia inner	16S2A	16S rDNA	AGTCAAACGGGATGTAGCAATAC	56	600–720	[14]
Borrelia inner	16S2B	16S rDNA	GTTATTCTTTCTGATATCAACAG	56	600–720	[14]

Abbreviation: T, temperature