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. 2019 Sep 11;14:307. doi: 10.1186/s13018-019-1307-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Effects of LPS on chondrocytes inflammation and collagen formation. a The mRNA levels of NRLP3, IL-1β, and IL-18 were analyzed by qRT-PCR in rat chondrocytes treated with LPS. b The release levels of IL-1β and IL-18 in the cell supernatant of LPS treated rat chondrocytes was checked by ELISA assay. c The expression level of collagen formation and pyroptosis-related protein was analyzed by Western blotting in rat chondrocytes treated with LPS. d Quantitative analysis of protein band gray in c. GAPDH was used as internal control. All the results were shown as mean ± SD (n = 3), which were three separate experiments performed in triplicate. *p < 0.05 and **p < 0.01