Table 1.
Kinetic parameters for the oxidation of p-methoxybenzyl alcohol and p-anisaldehyde by AAO in the presence of different H2O2 concentrations
| p-Methoxybenzyl alcohol | p-Anisaldehyde | ||||
|---|---|---|---|---|---|
| [H2O2] (mM) | kcat (s−1) | Km (µM) | kcat/Km (s−1mM−1) | [H2O2] (mM) | kcat (min−1) |
| 0 | 108 ± 3 | 31.8 ± 3.2 | 3390 ± 360 | 0 | 7.3 ± 0.7 |
| 0.5 | 115 ± 3 | 44.3 ± 3.5 | 2600 ± 220 | 1.0 | 7.3 ± 0.2 |
| 1.0 | 104 ± 3 | 33.5 ± 3.4 | 3100 ± 330 | 1.5 | 6.5 ± 0.3 |
| 1.9 | 109 ± 4 | 35.6 ± 4.3 | 3060 ± 390 | 3.1 | 6.7 ± 0.6 |
| 7.7 | 100 ± 4 | 33.7 ± 4.5 | 2970 ± 420 | 9.3 | 6.9 ± 0.5 |
| 15.5 | 97 ± 3 | 31.0 ± 2.6 | 3120 ± 280 | 12.3 | 6.9 ± 0.1 |
Activity was followed by measuring aldehyde formation (in p-methoxybenzyl alcohol oxidation) or acid formation (in p-anisaldehyde oxidation). Turnovers for aldehyde oxidation were calculated at saturating concentration of p-anisaldehyde (~ 5 mM) at 25 °C in air-saturated (0.256 mM O2) 50 mM sodium phosphate, pH 6.0