Table 2.
Kinetic parameters for the oxidation of p-methoxybenzyl alcohol by AAO in the presence of different FFCA concentrations
| [FFCA] (mM) | kcat (s−1) | Km (µM) | kcat/Km (s−1mM−1) |
|---|---|---|---|
| 0 | 84.5 ± 2.1 | 35.3 ± 2.8 | 2390 ± 200 |
| 0.4 | 68.0 ± 3.3 | 33.0 ± 5.0 | 2120 ± 350 |
| 0.8 | 78.9 ± 1.7 | 37.9 ± 2.5 | 2080 ± 140 |
| 1.5 | 73.8 ± 3.4 | 37.0 ± 5.1 | 1990 ± 290 |
| 3.0 | 66.9 ± 2.3 | 31.8 ± 3.6 | 2100 ± 250 |
| 6.0 | 61.9 ± 2.6 | 27.6 ± 2.6 | 2250 ± 220 |
Activity was followed by measuring the H2O2 released using AmplexRed® and HRP at 25 °C in air-saturated (0.256 mM O2) 50 mM sodium phosphate, pH 6.0