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. 2019 Sep 11;12:54. doi: 10.1186/s13072-019-0299-0

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Scheme of the model system. a Scheme of the inductor and the target T-DNAs; b expected activity of the T-DNAs in BY-2 cells untreated and treated with β-estradiol; P35S–CaMV constitutive promoter; XVE—a chimeric transcription activator; Pind—promoter activated by estradiol bound to XVE; HPT and NPTII, hygromycin, and kanamycin-resistance genes, respectively (for more detailed description, please see “Methods” and the original paper introducing the XVE-inducible system [70])