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. 2019 Sep 11;14:215. doi: 10.1186/s13023-019-1191-5

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© The Author(s). 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Results of whole-exome sequencing and Sanger sequencing of the patient with KLA (P1). a Integrative Genomics Viewer (IGV) view of NRAS c.182G > A in DNA samples from leukocytes (upper) and tumor mass (lower). c.182G > A allele was detected in 9 of 176 (5%) alleles. IGV shows reverse strands. b Results of Sanger sequencing of pTA cloning vectors. NRAS exon 2 was amplified by PCR in tumor DNA sample as a template. PCR product was subcloned into pTOPO cloning vector and each plasmid was sequenced. Six of sixty clones (10%) had a c.182G > A allele