Table 3.

Top 10 differentially methylation regions for 9-month control vs. pooled sequential/simultaneous

Region coordinates	Genes in region	Gene function [42]	# CpGs	FDR	Stouffer	β (max)	β (mean)
chr8:51306070–51306693	SNTG1	Mediates dystrophin binding. Associated with idiopathic scoliosis [49].	3	2.49E− 09	0.0904	0.2066	0.1012
chr3:150265617–150265620	SERP1	Protein folding and metabolism. Inhibition can downregulate the Wnt/β-catenin pathway in keratinocytes [70].	2	4.96E− 06	0.1754	0.0351	0.0327
chr3:44636619–44636844	ZNF197	Transcription factor with potential role in skeletal muscle energy metabolism following exercise [71].	2	0.0008	0.3556	0.0465	0.0267
chr22:49875144–49875295	C22orf34	Chromosome 22 Open Reading Frame 34, uncharacterized protein.	2	0.0040	0.3980	− 0.0775	− 0.0575
chr16:1477870–1478718	C16orf91	Chromosome 16 Open Reading Frame 91, uncharacterized protein.	3	0.0001	0.4290	− 0.0667	− 0.0434
chr19:7813422–7813963	EXOSC3P2	Pseudogene, unknown function.	3	3.81E− 06	0.4426	− 0.0800	− 0.0468
chr13:101173617–101174420	PCCA	Subunit of mitochondrial enzyme critical to energy metabolism [72].	8	4.01E− 11	0.4572	− 0.0929	− 0.0537
chr11:1903176–1903333	LSP1	Expressed in immune system cells and may regulate motility and transendothelial migration. Potential marker of hepatocellular carcinoma risk [73].	2	0.0047	0.4611	− 0.0586	− 0.0486
chr4:185369135–185370076	IRF2	Competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta. Decreased expression associated with angiogenesis and inflammation [43, 44].	3	0.0030	0.4752	− 0.1271	− 0.0100
chr4:175241684–175241753	CEP44	Centrosomal protein	2	0.0071	0.4980	− 0.1368	− 0.0847
chr17:72915897–72916509	USH1G	Auditory and visual function development [50].	3	0.0009	0.5005	− 0.0636	− 0.0595