Figure 3.

Ability of chondrocytes to outgrow in a biomaterial. (A) The scoring system. Top view of fibrin glue or CollPlant Vergenix gels at D28, stained by calcein/Hoechst. Blue, nuclei; green, live cells. Cartilage pieces are outlined in white and gels in yellow. White arrows indicate the presence of outgrown cells. Scale bars: 500 μm. Close-ups are shown underneath each image, outlined with dotted lines (scale bars: 100 µm). (B) Quantification of outgrowth. The mean ± SD score of the triplicates was used to obtain the final score for each condition for each patient. CollPlant, n = 11; fibrin glue, n = 12. (C) Sample images of 1 gel per condition. The related scores are indicated in the white frame, and arrows point at the presence of cells. Scale bars: 500 μm. Insets are shown underneath each image, outlined with dotted lines (scale bars: 100 µm). D28, after 28 days in culture.