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. 2019 Sep 4;10:999. doi: 10.3389/fphar.2019.00999

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Copyright © 2019 Zhao, Tian, Song, Ming, Liu, Shen, Liu and Yang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The G protein-PLC signal pathway is involved in neferine-induced GLUT4 expression in L6 cells. (A) G protein inhibitor gallein (100 µM) significantly inhibits neferine-induced GLUT4 mRNA expression. (B) Treatment with G protein inhibitor PTX (100 µM) significantly inhibits neferine-induced GLUT4 mRNA expression. (C) Ca²⁺ concentration induced by neferine was inhibited by G protein inhibitor gallein. (D) Ca²⁺ concentration induced by neferine was inhibited by G protein inhibitor PTX. (E) Significant inhibitory effect of the PLC inhibitor U73122 (1 µM) on neferine-induced GLUT4 mRNA expression. (F) U73343 inhibitor (inactive analogue of U73122, 10 µM) has no effect on neferine-induced GLUT4 mRNA expression. (G) U73122 inhibits the increase in Ca²⁺ concentration. (H) U73343 didn’t inhibit the increase in Ca²⁺ concentration. NS P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001.