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. Author manuscript; available in PMC: 2020 Sep 15.
Published in final edited form as: J Immunol. 2019 Aug 9;203(6):1502–1508. doi: 10.4049/jimmunol.1900819

Figure 3. Fluorescent H7-probe specifically detects H7-speciic GC B cells, which are not detected by an H1 specific probe.

Figure 3.

Mice were immunized subcutaneously in the footpad either with H7 protein or influenza nucleoprotein (NP) in alum. 14 days post-immunization, popliteal lymph nodes (pLN) were harvested and single cells analyzed by flow to detect H7-specific GC B cells. A) An APC-labeled H7- probe was used to identify H7-specific GC B cells. Representative staining is shown to illustrate the expression pattern of H7-specific GC B cells (CD4−CD19+B220hiCD138−GL−7+Fas+) in naïve mice, H7- immunized or NP-immunized mice as indicated in the top of the figures. H7 reactivity was only detected in the pLN from the H7 vaccinated mice. B) An APC-labeled H1-probe (A/California/04/09) was used in a separate aliquot of cells from the same cohorts of mice, showing the selectivity of binding.