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. 2019 Aug 22;146(16):dev175158. doi: 10.1242/dev.175158

Fig. 1.

Fig. 1.

Sexually dimorphic expression of abdominal apoptotic enhancer in Dsx+tNBs. (A) Schematic of terminal segments (A8-A11) of larval VNC. (B,C) Dsx+tNBs are marked by dsx-GAL4 and express AbdB in males (n=10) (B″) and females (n=11) (C″). In females, cell death was blocked by p35. (D) Blocking cell death from mid-L2 rescues Dsx+tNBs in female VNCs (see Fig. S2B for TS protocol, n=10). (E) F3B3-lacZ expresses in Dsx+tNBs of MM3 mutant female VNCs (n=11) until late L3. (F,G) F3B3-lacZ shows sex-specific expression in Dsx+tNBs of female VNCs (n=10) (G″) but not in male VNCs (n=11) (F″). (H) 717-lacZ is expressed in mid-L2, prior to apoptosis in Dsx+tNBs of female VNCs (n=12). (I) 717-lacZ sustains its expression in Dsx+tNBs in female VNCs until late L3 when apoptosis is blocked (Fig. S2A for TS protocol, n=8). n number of VNCs analysed. Yellow arrowheads indicate Dsx+tNBs. Scale bars: 10 µm. All images are single confocal sections.