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. 2019 Aug 22;146(16):dev175158. doi: 10.1242/dev.175158

Fig. 6.

Fig. 6.

DsxCR and AbdB cooperatively bind on the sex-specific apoptotic enhancer. (A) Schematic of F3B3-lacZ and 717-lacZ depicting six motifs containing Dsx and AbdB binding sites. Blue motifs exhibit cooperative binding of DsxCR and AbdB whereas green motifs do not (refer to Fig. S3). (B-D) EMSA for AbdB, DsxCR and AbdB-DsxCR binding on wild-type (WT), AbdB mutant and Dsx mutant oligos for motifs 2, 5 and 6. WT motif sequences are colour-coded for Hox (red) and Dsx (green) binding sites. Mutant variants are shown in lower case. Red arrowheads indicate Hox–DNA complex; green and dark green arrowheads indicate Dsx monomer and dimer complexes with DNA, respectively. Black arrowhead indicates Hox–Dsx–DNA cooperative complex. Grey arrowhead indicates cooperative complex with two molecules of Dsx. (B′-D′) Sequence comparison across multiple species for three motifs analysed. Dsx and Hox binding sites are highlighted by black and blue boxes, respectively.