Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 22;146(16):dev175158. doi: 10.1242/dev.175158

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 8. — Enhancer mutagenesis analysis in vivo. (A) Schematic of 717-lacZ, 717-Dsx^mutant-lacZ, 717-AbdB^mutant-lacZ and 717-Coop^mutant-lacZ. (B-F) Shows the enhancer-lacZ expression for wild-type and various mutant versions of the enhancer in Dsx⁺tNBs at mid-L2. 717-lacZ expresses normally (n=21) (B″) but Dsx^mutant-lacZ (n=14) (C″) and Coop^mutant-lacZ (n=12) (F″) fail to express in Dsx⁺tNBs of female VNCs in mid-L2. AbdB^mutant-lacZ expression is unaffected in Dsx⁺tNBs of females (n=9) (D″), but male VNCs show ectopic expression in Dsx⁺tNBs where it is normally not expressed (n=11) (E″). In contrast, Dsx^mutant-lacZ expression is not observed in either male (n=15) (G-G‴) or cell death-blocked female VNCs (n=8) (H-H″″) in late L3 (Fig. S2A for TS protocol). n indicates the number of VNCs analysed. Yellow arrowheads indicate Dsx⁺tNBs. Scale bars: 10 µm. All the images are single confocal sections.