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. 2019 Aug 23;132(16):jcs235507. doi: 10.1242/jcs.235507

Fig. 7.

Fig. 7.

Cell-associated MeV infection of neurons is N4 dependent. (A) Characterization of H358 N4-null SLAM+ cells. Analysis of N4 (left panel) and SLAM (right panel) surface expression. Color coding of cell lines: A431D, light blue; A431D N4–Dendra2, red; H358, magenta; H358 N4-null, orange; H358 N4-null SLAM+, green; VeroSLAM, black. (B) Fluorescent microscopy analysis of H358, H358 N4-null, and H358 N4-null SLAM+ cells 48 h post-infection with MeV-RNPtracker. (C) Time course of MeV–nCFP production by H358, H358 N4-null, and H358 N4-null SLAM+ cells (n=3, error bars, 3× s.d.). (D) Kinetic analysis of infection transfer by cells expressing or not expressing N4. Two experiments are plotted in the left and right panels. H358, H358 N4-null, or H358 N4-null SLAM+ cells were inoculated with MeV-RNPtracker. Vertical axis: number of fluorescence-emitting SCG cell bodies in the left compartment after overlay of neurites in the middle compartment. Each experiment used a minimum of six compartmentalized cultures seeded with infected cells. Results are mean±s.e.m. Asterisks indicate a non-zero number for H358 N4-null SLAM+ cells.