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. 2019 Jul 30;8(8):bio044024. doi: 10.1242/bio.044024

Fig. 4.

Fig. 4.

A fragment of Aip5 including the Bud6 binding site and the GRX-like domain rescues the LatA sensitivity of Δaip5-cells. (A) Wild-type, Δaip5, Δbud6 and Δaip5Δbud6 cells were incubated on SD plates in the presence of a centrally placed filter disk soaked with 10 µl LatA (500 µM). Values of the normalized area of growth inhibition were collected from four independent measurements (n=4) and compared by Kruskal–Wallis test and a Dunn’s post-test. (B) Δaip5 cells expressing different fragments of Aip5 CRU were incubated as in A in the presence of 250 µM LatA. The diameter of the zone of growth inhibition was measured and compared by one-way ANOVA and a Tukey’s post-test. Values were collected from three independent measurements (n=3). (C) Δaip5-cells expressing the indicated fragments of Aip5 as CRU fusions or an empty control plasmid were incubated as in A but on SD plates lacking methionine in the presence of 500 µM LatA. Values of the normalized area of growth inhibition were collected from three independent measurements (n=3) and compared by Kruskal–Wallis test and a Dunn’s post-test. Mean values with standard deviations are shown in red.