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. 2019 Aug 7;8(8):bio041293. doi: 10.1242/bio.041293

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — ROS generation in AC16 cells exposed to 50 Hz MF. Levels of intracellular ROS were measured by flow cytometry using DCFH-DA after 1 h continuous (A) or 75 min intermittent (B) exposure to 50 Hz MF. Cells treated with 200 μM H₂O₂ for 30 min were used as positive control. Left, representative cell DCF ﬂuorescence intensity profiles in negative control (control), sham exposed (sham), ELF-MF exposed (exposure) and positive control (positive) groups, from left to right. The vertical axis represents cell numbers, horizontal ordinate is DCF ﬂuorescence intensity. Right, histograms of geometric mean value of DCF ﬂuorescence intensity in each treatment group. The data represent the mean±s.e.m. from three independent experiments (**P<0.01 versus control).