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. 2019 Aug 15;8(8):bio042937. doi: 10.1242/bio.042937

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — miRNA-138 directly targets HIF1α and degrades its mRNA. (A) The HIF1α 3′UTR region containing the wild-type (WT) or mutant (Mut) binding site for miRNA-138. (B) The relative luciferase activity of HIF1α wild-type or mutant 3'UTR in HEK293 cells following transfection with miRNA-138 mimic or miRNA-control (Ctrl). (C–F) The HIF1α mRNA (C,E) and protein (D,F) expression in miRNA-138 knockdown (KD) WM35 cells (C,D) and miRNA-138 overexpressing (OE) A375 cells (E,F) was detected by qRT-PCR and western blot. **P<0.01, ***P<0.001 versus control.