Table 1.
Name | Primer sequences (5′–3′) | Application |
---|---|---|
ACTN4 3′UTR | F: TCTAGTTGTTTAAACGAGCTCGCCTCTTGCTCCCGTAAT R: CAGGTCGACTCTAGACTCGAGGGAGCAAAACCATCCACTA |
Amplification and vector construct |
PROX1 3′UTR | F: TCTAGTTGTTTAAACGAGCTCGTAGTCGCAGTCCCCTTT R: CAGGTCGACTCTAGACTCGAGTAAACTAAAGGCGGAAGG |
Amplification and vector construct |
ACTN4 3′UTR MT | F: TCTAGTTGTTTAAACGAGCTCTATGTTCTGAAATCGTAGTT R: CAGGTCGACTCTAGACTCGAGCCCTCCCTTGCGAACAC |
Mutation vector construct |
PROX1 3′UTR MT | F: TCTAGTTGTTTAAACGAGCTCTGGGTCTCTGAAAGTT R: CAGGTCGACTCTAGACTCGAGCCCCAGAAGGGTGGTTTATC |
Mutation vector construct |