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Experimental strategy to isolate EVs. 2 × 108 conidia/L were inoculated in YG media for 48, 72, 96, and 120 h at 37°C. After mycelium filtration with paper filter, the supernatant was filtered in a 0.45 μm membrane to retain reminiscent cells. Then, the flow-through was concentrated using a 100 kDa membrane. The supernatant was concentrated at 100,000 g to isolate the vesicles pellet.
