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. 2019 Sep 4;9:857. doi: 10.3389/fonc.2019.00857

Figure 5.

Figure 5

The apoptosis rate of CD3+ T cells and B16 melanoma cells in the co-cultured system. Cells were treated with anti-PD-1 antibody or vehicle for 12 h, and then harvested from the co-culture system and stained for flow cytometry. (A,B) The apoptosis rate of CD3+ T cells was higher when co-cultured with B16-c-FLIP-control cells than that with B16-c-FLIP-shRNA. (C) IFN-γ was markedly increased in the supernatant of the B16-c-FLIP-shRNA/lymphocytes co-culture system. Downregulation of c-FLIP increased apoptosis rate (D) and decreased the viability (E) in B16 melanoma cells. These experiments were repeated three times. LC: lymphocyte. *P < 0.05 (Student's t-test).