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. 2019 Sep 4;9:857. doi: 10.3389/fonc.2019.00857

Figure 6.

Figure 6

Downregulation of c-FLIPL could reduce PD-L1 expression in B16 melanoma cells. (A) The protein level of PD-L1 in melanoma cell lines was in accordance with the protein level of c-FLIPL. The protein expression of different groups and relative quantitative analysis results were labeled under the band. (B) The different level of PD-L1 expression was detected by immunofluorescence. The fluorescence intensity were quantitatively analyzed. Statistically differences between groups *P < 0.05 (Student's t-test). (C) Knockdown of c-FLIPL could downregulate the protein expression level of p-ERK, p-AKT, and PD-L1. (D) AKT inhibitor effectively suppressed p-AKT and PRAS40 in a dose-dependent manner, which resulted in a decrease of PD-L1 expression.