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. 2019 Sep 4;9:851. doi: 10.3389/fonc.2019.00851

Figure 2.

Figure 2

OLGEN Coverage Limit calculator—a simple theoretical calculator suitable for determining the correct sequencing depth and corresponding minimum number of variant reads according to the binomial distribution for a given sequencing error rate and intended LOD recommended for diagnostic NGS. Examples of calculated sequencing depths and the corresponding minimum number of variant reads recommended for variants with (A) 10% VAF and 99.9% probability of detection and (B) 3% VAF and 99.9% probability of detection.