Fig. 1.

H. diminuta extract (HdE) attenuates LPS-induced neutrophil recruitment to the knee. Lipopolysaccharide (LPS, 50 ng/10 μL) or sterile phosphate-buffered saline (PBS) were injected into the knee cavity (intra-articular [ia.]) ± co-treatment with HdE (1 mg/1 mL intraperitoneal [ip.]) or vehicle. Six hours later the knee cavity was washed to collect cells (a; representative of 3 experiments). Cells were also collected from the peritoneal cavity by washing (b), peripheral blood (c) and bone marrow from one femur (d). Neutrophils were identified by Wright-Giemsa staining of cytospin total cell preparations (data are mean ± SD; each datum point is an individual mouse; * and ^#, p < 0.05 compared to control [con] and LPS respectively, by one-way analysis of variance with Tukey's post-test).