Fig. 6.
A heat-, trypsin-sensitive glycoprotein mediates the effect of H. diminuta extract (HdE) on neutrophil chemotaxis. Murine bone marrow neutrophils (2 × 105 NØs) were treated with (a) boiled (boil), (b) trypsinized (tryp), (c) sodium metaperiodate (SMP) or (d) acidified (acid) HdE (100 μg/mL) and placed in the upper chamber of the transwell (8 μm pore size filter) separated from WKYMVm (1 μM) and NØ migration assessed 4 h later. Panel D shows the lack of an effect of applying polymyxin B (PmxB; 10 μg/mL) or E. coli-derived LPS (1 ng/mL) on the HdE-suppression of chemotaxis (data are mean ± SD; each datum point is the average of 2 NØ replicates from an individual mouse, * and # p < 0.05 compared to control (con) and WKYMVm only, respectively, by one-way analysis of variance with Tukey's post-test).